Effects Of Fixation On Tissues
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Effects Of Fixation On Tissues
ABSTRACT
Histology is the study of tissue. These tissues are processed in a variety of ways so that they can be thoroughly investigated. These procedures are referred to as histology techniques. Tissue processing can be done manually or with the help of an automated tissue processing machine (“tissue processor”).
Tissue processing schedules for enclosed automatic processors are designed based on tissue species and size/type. Most histological procedures have been devised to preserve the structural integrity of specimens so that they can be examined microscopically (Schwann, 2008).
The goal of appropriate histological technique is to maintain the microscopic anatomy of tissue and harden it so that very thin sections (4 to 5 microns) can be taken. Following staining, the section should represent the architecture of the tissue as closely as possible to its natural structure.
This is accomplished by subjecting the entire tissue as well as a specific portion of it to a number of processes. Light microscopy can be performed using three techniques: paraffin, frozen sections, and semi-thin sections.
The paraffin approach is the most widely employed (Preece, 2008).Fixation, dehydration, cleaning, embedding, cutting, and staining are histological methods used to prepare tissues for light microscopy.
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