Project Materials

FUNCTIONAL STATUS OF RENAL TISSUES OF ALLOXAN-INDUCED DIABETES MELLITUS RATS TREATED WITH MONOSODIUM GLUTAMATE/ASCORBIC ACID (200MG/4000MG)

Chapter one

INTRODUCTION

Background of the study

Diabetes mellitus is a chronic metabolic condition characterised by persistent hyperglycemia caused by insufficient insulin synthesis, insulin action, or both. Diabetic kidney disease (DKD) is one of the most prevalent and serious consequences of diabetes, affecting a large number of diabetics. DKD is a prominent cause of end-stage renal disease (ESRD), resulting in significant morbidity and mortality.

Oxidative stress, inflammation, and impaired renal function are important factors in the development and progression of DKD. Hyperglycemia-induced oxidative stress produces reactive oxygen species (ROS), which can harm the kidneys by affecting cellular function and causing inflammation. As a result, therapies that target oxidative stress while also improving renal function are highly sought after in DKD care.

Monosodium glutamate (MSG), a flavour enhancer often used in the food business, has been studied for potential therapeutic effects in a variety of conditions, including diabetes. It has been shown to have antioxidant, anti-inflammatory, and antidiabetic effects.

Ascorbic acid (AA), generally known as vitamin C, is a powerful antioxidant that has been demonstrated to preserve kidneys in DKD models.

Given the possible benefits of MSG and AA individually, it is worthwhile to study their combined therapeutic potential in DKD.

The purpose of this study was to assess the functional condition of renal tissues in alloxan-induced diabetes mellitus rats treated with a combination of MSG and AA (200mg/4000mg) for 28 days.

Alloxan-induced diabetes mellitus rat models are frequently employed in preclinical research to mimic the metabolic and pathophysiological alterations seen in human diabetes.

Understanding the effects of MSG/AA combination therapy on renal function metrics, oxidative stress indicators, and histological alterations in renal tissues may shed light on its potential as a therapeutic intervention for DKD. The outcomes of this study may help to create novel treatment techniques to reduce kidney problems in diabetics.

In summary, the purpose of this study is to evaluate the functional condition of renal tissues in alloxan-induced diabetes mellitus rats treated with MSG/AA combination therapy.

The study will give important information about the efficacy of this treatment technique in improving renal dysfunction and oxidative stress, underlining its potential as a therapeutic intervention for DKD.

Statement of the Problem

Diabetes mellitus is a chronic metabolic illness characterised by hyperglycemia and other consequences, including diabetic kidney disease (DKD). DKD is a primary cause of end-stage renal disease (ESRD), and it poses